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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
29/10/2019 |
Actualizado : |
12/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
URRABURU, M.; AGUAYO, E.; ZOPPOLO, R.; SILVEIRA, A.C. |
Afiliación : |
MARIANA URRABURU BORDON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. AGUAYO, Grupo de Postrecoleccio?n y Refrigeracio?n, Universidad Polite?cnica de Cartagena, Spain; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A.C. SILVEIRA, Poscosecha de Frutas y Hortalizas, Departamento de Produccio?n Vegetal, Facultad de Agronom??a, Universidad de La Repu?blica, Uruguay. |
Título : |
Postharvest and functional behavior of some feijoa genetic materials. (Conference paper). |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Acta Horticulturae, 2018, no. 1194, pages 73-78. |
Serie : |
(Acta Horticulturae; 1194) |
ISBN : |
978-94-62611-90-0 |
ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2018.1194.12 |
Idioma : |
Inglés |
Notas : |
In: Acta Horticulturae (ISHS) 1194: VIII International Postharvest Symposium: Enhancing Supply Chain and Consumer Benefits - Ethical and Technological Issues. Editors: F. Artés-Hernández, P.A. Gómez, E. Aguayo, F. Artés. Publication date: 4 april 2018. |
Contenido : |
ABSTRACT.
Feijoa (Acca sellowiana (Berg.) Burret) is one of the most popular native Uruguayan trees whose functional characteristics have attracted the interest for commercial cultivation. The aim of this work was to evaluate the postharvest behavior of six genetic materials, identified as 95, 97, 98, 152, 153 and 154 from the Facultad de Agronomía and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) breeding program. Fruits harvested at two maturity stages (M1: not easily picked; M2: touch picking) were stored for 0, 15 and 30 days at 5°C and 95% relative humidity (RH) plus 7 days at 18°C (shelf life). Respiration rates and ethylene emissions were measured every 3 days, while flesh firmness, weight loss and total polyphenol content were evaluated at the end of the storage period and during shelf life. The respiration rate of all the genetic materials was around 27.6 mg kg-1 h-1 of CO2 during the storage period, while ethylene emission was maintained below 100 µL kg-1 h-1 without differences among maturity stages and genetic materials. Fruits harvested at the M1 stage were 40-60% more firm than fruits harvested at the M2 stage. Genetic materials identified as 95 and 98 had the highest firmness results, with values of 150.1 and 145.1 N (M1), 112.6 and 90.4 N (M2) at harvest and 28.8 and 17.4 N (M1) and 17.2 and 15.4 N (M2) after 30 days at 5°C + 7 days at 20°C, respectively. Average weight loss at the end of storage (30+7) was 14.7%. Fruits identified as material 152 had the highest polyphenol content with values at harvest of 2,384.5 mg GAE 100 g-1 DW (M1) and 2,130.7 mg GAE 100 g-1 DW (M2). At the end of the storage period (30+7), total polyphenol contents decreased between 30-70% in the M1 stage and between 20-50% in the M2 stage. Materials 95, 98 and 152 are interesting for commercial production due to their firmness retention and their functional composition.
© 2018 International Society for Horticultural Science. All rights reserved MenosABSTRACT.
Feijoa (Acca sellowiana (Berg.) Burret) is one of the most popular native Uruguayan trees whose functional characteristics have attracted the interest for commercial cultivation. The aim of this work was to evaluate the postharvest behavior of six genetic materials, identified as 95, 97, 98, 152, 153 and 154 from the Facultad de Agronomía and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) breeding program. Fruits harvested at two maturity stages (M1: not easily picked; M2: touch picking) were stored for 0, 15 and 30 days at 5°C and 95% relative humidity (RH) plus 7 days at 18°C (shelf life). Respiration rates and ethylene emissions were measured every 3 days, while flesh firmness, weight loss and total polyphenol content were evaluated at the end of the storage period and during shelf life. The respiration rate of all the genetic materials was around 27.6 mg kg-1 h-1 of CO2 during the storage period, while ethylene emission was maintained below 100 µL kg-1 h-1 without differences among maturity stages and genetic materials. Fruits harvested at the M1 stage were 40-60% more firm than fruits harvested at the M2 stage. Genetic materials identified as 95 and 98 had the highest firmness results, with values of 150.1 and 145.1 N (M1), 112.6 and 90.4 N (M2) at harvest and 28.8 and 17.4 N (M1) and 17.2 and 15.4 N (M2) after 30 days at 5°C + 7 days at 20°C, respectively. Average weight loss at the end of storage (30+7) was 14.7%. Fruits identified as mater... Presentar Todo |
Palabras claves : |
Ethylene; Firmness; Functional quality; Respiration. |
Thesagro : |
ACCA SELLOWIANA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 03073naa a2200277 a 4500 001 1060362 005 2019-11-12 008 2018 bl uuuu u00u1 u #d 020 $a978-94-62611-90-0 022 $a0567-7572 (print) / 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2018.1194.12$2DOI 100 1 $aURRABURU, M. 245 $aPostharvest and functional behavior of some feijoa genetic materials. (Conference paper).$h[electronic resource] 260 $c2018 490 $a(Acta Horticulturae; 1194) 500 $aIn: Acta Horticulturae (ISHS) 1194: VIII International Postharvest Symposium: Enhancing Supply Chain and Consumer Benefits - Ethical and Technological Issues. Editors: F. Artés-Hernández, P.A. Gómez, E. Aguayo, F. Artés. Publication date: 4 april 2018. 520 $aABSTRACT. Feijoa (Acca sellowiana (Berg.) Burret) is one of the most popular native Uruguayan trees whose functional characteristics have attracted the interest for commercial cultivation. The aim of this work was to evaluate the postharvest behavior of six genetic materials, identified as 95, 97, 98, 152, 153 and 154 from the Facultad de Agronomía and Instituto Nacional de Investigación Agropecuaria (INIA, Uruguay) breeding program. Fruits harvested at two maturity stages (M1: not easily picked; M2: touch picking) were stored for 0, 15 and 30 days at 5°C and 95% relative humidity (RH) plus 7 days at 18°C (shelf life). Respiration rates and ethylene emissions were measured every 3 days, while flesh firmness, weight loss and total polyphenol content were evaluated at the end of the storage period and during shelf life. The respiration rate of all the genetic materials was around 27.6 mg kg-1 h-1 of CO2 during the storage period, while ethylene emission was maintained below 100 µL kg-1 h-1 without differences among maturity stages and genetic materials. Fruits harvested at the M1 stage were 40-60% more firm than fruits harvested at the M2 stage. Genetic materials identified as 95 and 98 had the highest firmness results, with values of 150.1 and 145.1 N (M1), 112.6 and 90.4 N (M2) at harvest and 28.8 and 17.4 N (M1) and 17.2 and 15.4 N (M2) after 30 days at 5°C + 7 days at 20°C, respectively. Average weight loss at the end of storage (30+7) was 14.7%. Fruits identified as material 152 had the highest polyphenol content with values at harvest of 2,384.5 mg GAE 100 g-1 DW (M1) and 2,130.7 mg GAE 100 g-1 DW (M2). At the end of the storage period (30+7), total polyphenol contents decreased between 30-70% in the M1 stage and between 20-50% in the M2 stage. Materials 95, 98 and 152 are interesting for commercial production due to their firmness retention and their functional composition. © 2018 International Society for Horticultural Science. All rights reserved 650 $aACCA SELLOWIANA 653 $aEthylene 653 $aFirmness 653 $aFunctional quality 653 $aRespiration 700 1 $aAGUAYO, E. 700 1 $aZOPPOLO, R. 700 1 $aSILVEIRA, A.C. 773 $tActa Horticulturae, 2018, no. 1194, pages 73-78.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
10/11/2023 |
Actualizado : |
10/11/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
ISSN : |
1642-431X |
DOI : |
10.1016/j.repbio.2023.100814 |
Idioma : |
Inglés |
Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
Asunto categoría : |
L50 Fisiología y bioquímica animal |
Marc : |
LEADER 03765naa a2200301 a 4500 001 1064365 005 2023-11-10 008 2023 bl uuuu u00u1 u #d 022 $a1642-431X 024 7 $a10.1016/j.repbio.2023.100814$2DOI 100 1 $aBORGES, M.A. 245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- 520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn 653 $aFixative solution 653 $aMare 653 $aOvarian tissue quality 653 $aPreantral follicle morphometry 700 1 $aCURCIO, B.R. 700 1 $aGASTAL, G.D.A. 700 1 $aGHENO, L. 700 1 $aJUNIOR, A.S.V. 700 1 $aCORCINI, C.D. 700 1 $aNOGUEIRA, C.E.W. 700 1 $aAGUIAR, F.L.N. 700 1 $aGASTAL, E.L. 773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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